Dr. Oliver Lenz

Production of biohydrogen

Research interests

Structure, function and application of oxygen tolerant [Ni-Fe]-hydrogenases

Research Fields in UniCat

Research Field E3

2007-2011: B1, B2


Selection of research projects

Structure-function relationship of O2-tolerant hydrogenases
Cofactor assembly and maturation of [NiFe]-hydrogenases
O2-tolerant hydrogenases in biotechnology

UniCat publications

799 NAD(H)-coupled hydrogen cycling - structure-function relationships of bidirectional [NiFe] hydrogenases M. Horch, L. Lauterbach, O. Lenz, P. Hildebrandt, I. Zebger FEBS letters 2012, 586, 545 - 556, 10.1016/j.febslet.2011.10.010

792 Systematic evaluation of the dihydrogen-oxidising and NAD+-reducing [NiFe] hydrogenase from Ralstonia eutropha H16 as a cofactor regeneration catalyst J. Ratzka, L. Lauterbach, O. Lenz, M. Ansorge-Schumacher Biocatalysis & Biotransformation 2011, 29, 246 - 252, 10.3109/10242422.2011.615393

782 A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties H. A. Reeve, L. Lauterbach, P. A. Ash, O. Lenz, K. A. Vincent Chemical Communications 2012, 48, 1589 - 1591, 10.1039/C1CC14826E

780 Probing the origin of the metabolic precursor of the CO ligand in the catalytic center of [NiFe]-hydrogenase I. Bürstel, P. Hummel, E. Siebert, N. Wisitruangsakul, I. Zebger, B. Friedrich, O. Lenz Journal of Biological Chemistry 2011, 286, 44937 - 44944, 10.1074/jbc.M111.309351

775 A Trimeric Supercomplex of the Oxygen-Tolerant Membrane-Bound [NiFe]-Hydrogenase from Ralstonia eutropha H16 S. Frielingsdorf, T. Schubert, A. Pohlmann, O. Lenz, B. Friedrich Biochemistry 2011, 50, 10836 - 10843, 10.1021/bi201594m

763 Catalytic Properties of the Isolated Diaphorase Fragment of the NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha L. Lauterbach, Z. Idris, K. A. Vincent, O. Lenz PLoS ONE 2011, 6, 0 - 0, 10.1371/journal.pone.0025939

762 Oxygen-Tolerant [NiFe]-Hydrogenases: The Individual and Collective Importance of Supernumerary Cysteines at the Proximal Fe-S Cluster M. J. Lukey, M. M. Roessler, A. Parkin, R. M. Evans, R. A. Davies, O. Lenz, B. Friedrich, F. Sargent, F. A. Armstrong Journal of the American Chemical Society 2011, 133, 16881 - 16892, dx.doi.org/10.1021/ja205393w

761 Contribution of a Sodium Ion Gradient to Energy Conservation during Fermentation in the Cyanobacterium Arthrospira (Spirulina) maxima CS-328 D. Carrieri, G. Ananyev, O. Lenz, D. A. Bryant, G. C. Dismukes Applied and Environmental Microbiology 2011, 77, 7185 - 7194, 10.1128/AEM.00612-11

760 [NiFe] and [FeS] Cofactors in the Membrane-Bound Hydrogenase of Ralstonia eutropha Investigated by X-ray Absorption Spectroscopy: Insights into O2-Tolerant H2 Cleavage F. Johannes, S. Löscher, O. Sanganas, E. Siebert, I. Zebger, M. Stein, M. Ludwig, A. L. De Lacey, H. Dau, B. Friedrich, O. Lenz, M. Haumann Biochemistry 2011, 50, 5858 - 5869, dx.doi.org/10.1021/bi200367u

751 Stabilisation of the NAD+-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 through modification with methoxy-poly(methylene) glycol J. Ratzka, L. Lauterbach, O. Lenz, M. Ansorge-Schumacher Journal of Molecular Catalysis B: Enzymatic 2012, 74, 219 - 223, 10.1016/j.molcatb.2011.10.008

728 The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre J. Fritsch, P. Scheerer, S. Frielingsdorf, S. Kroschinsky, B. Friedrich, O. Lenz, C. Spahn Nature 2011, 479, 249 - 252, 10.1038/nature10505

665 Oxygen-tolerant hydrogenases in hydrogen-based technologies B. Friedrich, J. Fritsch, O. Lenz Current Opinion in Biotechnology 2011, 22, 358 - 364, 10.1016/j.copbio.2011.01.006

578 The maturation factors HoxR and HoxT contribute to oxygen tolerance of membrane-bound [NiFe] hydrogenase in Ralstonia eutropha H16 J. Fritsch, O. Lenz, B. Friedrich Journal of Bacteriology 2011, 193, 2487 - 2497, 10.1128/JB.01427-10

577 A unique iron-sulfur cluster is crucial for oxygen tolerance of a [NiFe]-hydrogenase T. Goris, A. F. Wait, M. Saggu, J. Fritsch, N. Heidary, M. Stein, I. Zebger, F. Lendzian, F. A. Armstrong, B. Friedrich, O. Lenz Nature Chemical Biology 2011, 7, 310 - 318, 10.1038/NCHEMBIO.555

491 Probing the active site of an oxygen-tolerant NAD+ reducing [NiFe] hydrogenase from Ralstonia eutropha H16 by in-situ EPR and FTIR spectroscopy M. Horch, L. Lauterbach, M. Saggu, O. Lenz, P. Hildebrandt, F. Lendzian, R. Bittl, Z. Ingo Angewandte Chemie International Edition 2010, 49, 8026 - 8029, 10.1002/anie.201002197

458 The Hydrogenase Subcomplex of the NAD+-Reducing [NiFe] Hydrogenase from Ralstonia eutropha - Insights into Catalysis and Redox Interconversions L. Lauterbach, J. Liu, M. Horch, P. Hummel, A. Schwarze, M. Haumann, K. A. Vincent, O. Lenz, I. Zebger European Journal of Inorganic Chemistry 2011, 7, 1067 - 1079, 10.1002/ejic.201001053

456 Oxygen-tolerant hydrogenases in hydrogen-based technologies B. Friedrich, J. Fritsch, O. Lenz Current Opinion in Biotechnology 2011, 22, 358 - 364, 10.1016/j.copbio.2011.01.006

450 Boosting Autofermentation Rates and Product Yields with Sodium Stress Cycling: Application to Production of Renewable Fuels by Cyanobacteria D. Carrieri, D. Momot, I. A. Brasg, G. Ananyev, O. Lenz, D. A. Bryant, G. C. Dismukes Applied and Environmental Microbiology 2010, 76, 6455 - 6462, 10.1128/AEM.00975-10

355 H2 conversion in the presence of O2 as performed by the membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha O. Lenz, M. Ludwig, T. Schubert, I. Buerstel, S. Ganskow, T. Goris, A. Schwarze, B. Friedrich ChemPhysChem 2010, 11, 1107 - 1119, 10.1002/cphc.200901002

349 Impact of amino acid substitutions near the catalytic site on the spectral properties of an O2-tolerant membrane-bound [NiFe]-hydrogenase M. Saggu, M. Ludwig, B. Friedrich, P. Hildebrandt, R. Bittl, F. Lendzian, O. Lenz, I. Zebger ChemPhysChem 2010, 11, 1215 - 1224, 10.1002/cphc.200900988

348 Photosynthetic hydrogen production by a hybrid complex of photosystem I and [NiFe]-hydrogenase H. Krassen, A. Schwarze, B. Friedrich, K. Ataka, O. Lenz, J. Heberle ACS Nano 2009, 3, 4055 - 4061, 10.1021/nn900748j

347 A kinetic and thermodynamic understanding of O2 tolerance in [NiFe]-hydrogenases J. A. Cracknell, A. F. Wait, O. Lenz, B. Friedrich, F. A. Armstrong Proceedings of the National Academy of Sciences of the United States of America 2009, 106, 20681 - 20686, 10.1073/pnas.0905959106

343 Requirements for the construction of a functional hybrid complex between photosystem I and [NiFe]-hydrogenase A. Schwarze, M. Kopczak, M. Rögner, O. Lenz Applied and Environmental Microbiology 2010, 76, 2641 - 2651, 10.1128/AEM.02700-09

136 Enzymatic oxidation of H2 in atmospheric O2: The electrochemistry of energy generation from trace H2 by aerobic by aerobic microorganisms J. A. Cracknell, K. A. Vincent, M. Ludwig, O. Lenz, B. Friedrich, F. A. Armstrong Journal of the American Chemical Society 2008, 130, 424 - 425, 10.1021/ja078299

135 Hydrogen production under aerobic conditions by membrane-bound hydrogenases from Ralstonia species G. Goldet, A. F. Wait, J. A. Cracknell, K. A. Vincent, M. Ludwig, O. Lenz, B. Friedrich, F. A. Armstrong Journal of the American Chemical Society 2008, 130, 11106 - 11113, 10.1021/ja8027668

133 Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe]-hydrogenase M. Ludwig, T. Schubert, I. Zebger, N. Wisitruangsakul, M. Saggu, A. Strack, O. Lenz, P. Hildebrandt, B. Friedrich The Journal Of Biological Chemistry 2009, 284, 2159 - 2168, 10.1074/jbc.M808488200

131 Oxygen-tolerant H2 oxidation by membrane-bound [NiFe]-hydrogenase of Ralstonia species: Coping with low-level H2 in air M. Ludwig, J. A. Cracknell, K. A. Vincent, F. A. Armstrong, O. Lenz The Journal Of Biological Chemistry 2009, 284, 465 - 477, 10.1074/jbc.M803676200

130 Knallgas unter Kontrolle: O2-tolerante Hydrogenasen und ihre Anwendung M. Ludwig, A. Schwarze, O. Lenz BIOspektrum 2008, 5, 477 - 479,

129 Biokatalysatoren für die Erzeugung von Wasserstoff und Strom O. Lenz, B. Friedrich Aktuelle Wochenschau (Online) 2008, 0, 0 - 0,

52 Spectroscopic insight into the oxygen-tolerant membrane-associated [NiFe]-hydrogenase of Ralstonia eutropha H16 M. Saggu, I. Zebger, M. Ludwig, O. Lenz, B. Friedrich, P. Hildebrandt, F. Lendzian Journal of Biological Chemistry 2009, 284, 16264 - 16276, 10.1074/jbc.M805690200

49 Untersuchung des Katalyseprozesses der membrangebundenen Hydrogenase aus Rastolnia EutrophaH16 mittels oberflächenverstärkter Infrarotabsorptionsspektroskopie N. Wisitruangsakul, O. Lenz, M. Ludwig, B. Friedrich, P. Hildebrandt, F. Lendzian, I. Zebger Angewandte Chemie 2009, 121, 621 - 623, 10.1002/ange.200802633

48 Monitoring catalysis of the membrane-bound hydrogenase of Rastolnia Eutropha by surface enhanced infrared absorption spectroscopy N. Wisitruangsakul, O. Lenz, M. Ludwig, B. Friedrich, P. Hildebrandt, F. Lendzian, I. Zebger Angewandte Chemie International Edition 2009, 48, 611 - 613, 10.1002/anie.200802633

Dr. Oliver Lenz

Photo of Oliver  Lenz

Oliver Lenz
Humboldt-Universität zu Berlin
Institut für Biologie
Chausseestraße 117
10115 Berlin

+49 (0)30 2093-8173
oliver.lenz(at)rz.hu-berlin.de

 

 

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