In vivo double and triple labeling of proteins using synthetic amino acids

Thursday, 24. June 2010

The group of Prof. Budisa could demonstrate that it is possible to introduce three types of non-canonical amino acids into a protein in a single expression experiment.

The use of non-canonical amino acids in protein engineering gained much attention in recent years. Genetic code engineering is based on in vivo sense-codon reassignment, whereby synthetic amino acids are translated into protein sequences in residue-specific manner. In this way proteins are chemically enriched in a controlled manner without need for DNA mutagenesis. But until now, only one type of non-canonical amino acids could be introduced into protein sequences...

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You can find the full article of Nediljko Budisa and coworkers at  Research\Research Highlights.