Prof. Dr. Nediljko Budisa - Publications

2322 Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence T. Baumann, F. J. Schmitt, A. Pelzer, V. J. Spiering, G. J. Freiherr von Sass, T. Friedrich, N. Budisa Journal of Visualized Experiments 2018, 10.3791/57017

2320 Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids J. H. Nickling, T. Baumann, F. J. Schmitt, M. Bartholomae, O. P. Kuipers, T. Friedrich, N. Budisa Journal of Visualized Experiments 2018, 10.3791/57551

2279 Prospects of In vivo Incorporation of Non-canonical Amino Acids for the Chemical Diversification of Antimicrobial Peptides T. Baumann, J. H. Nickling, M. Bartholomae, A. Buivydas, O. P. Kuipers, N. Budisa Frontiers in Microbiology 2017, 8, 10.3389/fmicb.2017.00124

2278 Global substitution of hemeproteins with noncanonical amino acids inEscherichia coliwith intact cofactor maturation machinery J. S. Völler, T. M. Thi To, H. Biava, B. Koksch, N. Budisa Enzyme and Microbial Technology 2017, 106, 55–59, 10.1016/j.enzmictec.2017.06.014

2277 Peptidyl-Prolyl Model Study: How Does the Electronic Effect Influence the Amide Bond Conformation? P. K. Mykhailiuk, V. Kubyshkin, T. Bach, N. Budisa The Journal of Organic Chemistry 2017, 82, 8831–8841, 10.1021/acs.joc.7b00803

2276 The Regioselective Synthesis of o-Nitrobenzyl DOPA Derivatives T. Schneider, J. Martin, P. Durkin, V. Kubyshkin, N. Budisa Synthesis 2017, 49, 2691–2699, 10.1055/s-0036-1588766

2275 Hydrolysis, polarity, and conformational impact of C-terminal partially fluorinated ethyl esters in peptide models V. Kubyshkin, N. Budisa Beilstein Journal of Organic Chemistry 2017, 13, 2442–2457, 10.3762/bjoc.13.241

2274 Amide rotation trajectories probed by symmetry V. Kubyshkin, N. Budisa Organic & Biomolecular Chemistry 2017, 15, 6764–6772, 10.1039/C7OB01421J

2273 Selective19F-Labeling of Functionalized Carboxylic Acids with Difluoromethyl Diazomethane (CF2HCHN2) P. K. Mykhailiuk, I. Kishko, V. Kubyshkin, N. Budisa, J. Cossy Chemistry - A European Journal 2017, 23, 13279–13283, 10.1002/chem.201703446

2272 Deciphering the Fluorine Code—The Many Hats Fluorine Wears in a Protein Environment A. A. Berger, J. S. Völler, N. Budisa, B. Koksch Accounts of Chemical Research 2017, 50, 2093–2103, 10.1021/acs.accounts.7b00226

2271 Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology F. Agostini, J. S. Völler, B. Koksch, C. G. Acevedo-Rocha, V. Kubyshkin, N. Budisa Angewandte Chemie International Edition 2017, 56, 9680–9703, 10.1002/anie.201610129

2169 An expanded genetic code for probing the role of electrostatics in enzyme catalysis by vibrational Stark spectroscopy J. S. Völler, H. Biava, P. Hildebrandt, N. Budisa Biochimica et Biophysica Acta (BBA) - General Subjects 2017, 1861, 3053–3059, 10.1016/j.bbagen.2017.02.009

2076 Discovery and Investigation of Natural Editing Function against Artificial Amino Acids in Protein Translation J. S. Völler, M. Dulic, U. I. M. Gerling-Driessen, H. Biava, T. Baumann, N. Budisa, I. Gruic-Sovulj, B. Koksch ACS Central Science 2017, 3, 73–80, 10.1021/acscentsci.6b00339

2047 cistrans-Amide isomerism of the 3,4-dehydroproline residue, the ‘unpuckered’ proline V. Kubyshkin, N. Budisa Beilstein Journal of Organic Chemistry 2016, 12, 589–593, 10.3762/bjoc.12.57

2046 Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis T. Baumann, M. Exner, N. Budisa Advances in Biochemical Engineering/Biotechnology Springer International Publishing Switzerland Springer Berlin Heidelberg 2016,

2044 Energetic contribution to both acidity and conformational stability in peptide models V. Kubyshkin, P. Durkin, N. Budisa New J. Chem. 2016, 40, 5209–5220, 10.1039/C5NJ03611A

2043 Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins M. Exner, S. Köhling, J. Rivollier, S. Gosling, P. Srivastava, Z. Palyancheva, P. Herdewijn, M. P. Heck, J. Rademann, N. Budisa Molecules 2016, 21, 287– 10.3390/molecules21030287

2042 Congeneric lipases with improved catalytic activity and substrate accessibility / Engineering lipases with an expanded genetic code. A. De Simone, M. G. Hoesl, N. Budisa Applied Biocatalysis - From Fundamental Science to Industrial Applications Garabed Antranikian Wiley-VCH 2016,

2041 Construction of a polyproline structure with hydrophobic exterior using octahydroindole-2-carboxylic acid V. Kubyshkin, N. Budisa Org. Biomol. Chem. 2017, 15, 619–627, 10.1039/C6OB02306A

2040 One-Pot Synthesis of Unprotected Anomeric Glycosyl Thiols in Water for Glycan Ligation Reactions with Highly Functionalized Sugars S. Köhling, M. P. Exner, S. Nojoumi, J. Schiller, N. Budisa, J. Rademann Angewandte Chemie International Edition 2016, 55, 15510–15514, 10.1002/anie.201607228

2039 Design ofS-Allylcysteine in Situ Production and Incorporation Based on a Novel Pyrrolysyl-tRNA Synthetase Variant M. P. Exner, T. Kuenzl, T. M. T. To, Z. Ouyang, S. Schwagerus, M. G. Hoesl, C. P. R. Hackenberger, M. C. Lensen, S. Panke, N. Budisa ChemBioChem 2016, 18, 85–90, 10.1002/cbic.201600537

2005 Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coli Strain Produces a New-to-nature Bioactive Lantibiotic Containing Thienopyrrole-Alanine A. Kuthning, P. Durkin, S. Oehm, M. G. Hoesl, N. Budisa, R. D. Süssmuth Scientific Reports 2016, 6, 33447– 10.1038/srep33447

1750 Toward intrinsically colored peptides: Synthesis and investigation of the spectral properties of methylated azatryptophans in tryptophan-cage mutants B. P. Noichl, P. M. Durkin, N. Budisa Biopolymers 2015, 104, 585–600, 10.1002/bip.22709

1749 Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids Y. Ma, B. N. S. Thota, R. Haag, N. Budisa Bioorganic & Medicinal Chemistry Letters 2015, 25, 5247–5249, 10.1016/j.bmcl.2015.09.055

1748 Chemical Evolution of a Bacterial Proteome M. G. Hoesl, S. Oehm, P. Durkin, E. Darmon, L. Peil, H. R. Aerni, J. Rappsilber, J. Rinehart, D. Leach, D. Söll, N. Budisa Angewandte Chemie International Edition 2015, 54, 10030–10034, 10.1002/anie.201502868

1747 Chemische Evolution eines bakteriellen Proteoms M. G. Hoesl, S. Oehm, P. Durkin, E. Darmon, L. Peil, H. R. Aerni, J. Rappsilber, J. Rinehart, D. Leach, D. Söll, N. Budisa Angewandte Chemie 2015, 127, 10168–10172, 10.1002/ange.201502868

1746 Entropic Contribution of Elongation Factor P to Proline Positioning at the Catalytic Center of the Ribosome L. K. Doerfel, I. Wohlgemuth, V. Kubyshkin, A. L. Starosta, D. N. Wilson, N. Budisa, M. V. Rodnina Journal of the American Chemical Society 2015, 137, 12997–13006, 10.1021/jacs.5b07427

1668 Orthogonal Translation Meets Electron Transfer: In Vivo Labeling of Cytochrome c for Probing Local Electric Fields J. Völler, H. Biava, B. Koksch, P. Hildebrandt, N. Budisa ChemBioChem 2015, 16, 742–745, 10.1002/cbic.201500022

1587 Applying γ-Substituted Prolines in the Peptide: Polarity Contradicts Preorganization D. Dietz, V. Kubyshkin, N. Budisa ChemBioChem 2015, 16, 403–406, 10.1002/cbic.201402654

1586 Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability N. Nischan, H. D. Herce, F. Natale, N. Bohlke, N. Budisa, M. C. Cardoso, C. P. R. Hackenberger Angewandte Chemie International Edition 2015, 54, 1950–1953, 10.1002/anie.201410006

1582 Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins Y. Ma, H. Biava, R. Contestabile, N. Budisa, M. di Salvo Molecules 2014, 19, 1004–1022, 10.3390/molecules19011004

1581 Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion N. Bohlke, N. Budisa FEMS Microbiology Letters 2014, 351, 133–144, 10.1111/1574-6968.12371

1579 Evolution of fluorinated enzymes: An emerging trend for biocatalyst stabilization H. Biava, N. Budisa Engineering in Life Sciences 2014, 14, 340–351, 10.1002/elsc.201300049

1552 Site-Directed and Global Incorporation of Orthogonal and Isostructural Noncanonical Amino Acids into the Ribosomal Lasso Peptide Capistruin R. S. Al Toma, A. Kuthning, M. P. Exner, A. Denisiuk, J. Ziegler, N. Budisa, R. D. Süssmuth ChemBioChem 2015, 16, 503–509, 10.1002/cbic.201402558

1340 PLP-dependent Enzymes: a Powerful Tool for Metabolic Synthesis of. Non-canonical Amino Acids M. Si Salvo, N. Budisa, R. Contestabile Beilstein Symposium on Molecular Engineering and Control M. G. Hicks and Kettner, C. Logos Verlag 2014,

1339 Expanded genetic code for the engineering of ribosomally synthetized and post-translationally modified peptide natural products (RiPPs) N. Budisa Current Opinion in Biotechnology 2013, 24, 591–598, 10.1016/j.copbio.2013.02.026

1338 Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments C. G. Acevedo-Rocha, M. G. Hoesl, S. Nehring, M. Royter, C. Wolschner, B. Wiltschi, G. Antranikian, N. Budisa Catalysis Science & Technology 2013, 3, 1198– 10.1039/c3cy20712a

1337 Biocatalytic synthesis of (2S)-5,5,5-trifluoroleucine and improved resolution into (2S,4S) and (2S,4R) diastereoisomers H. Biava, N. Budisa Tetrahedron Letters 2013, 54, 3662–3665, 10.1016/j.tetlet.2013.04.128

1336 Obtention of enantiomerically pure 5,5,5-trifluoro-l-isoleucine and 5,5,5-trifluoro-l-alloisoleucine H. Biava, N. Budisa Journal of Fluorine Chemistry 2013, 156, 372–377, 10.1016/j.jfluchem.2013.07.021

836 Congeneric Lantibiotics from Ribosomal In Vivo Peptide Synthesis with Noncanonical Amino Acids** F. Oldach, R. Al Toma, A. Kuthning, T. Caetano, S. Mendo, N. Budisa, R. Süssmuth Angewandte Chemie International Edition 2012, 51, 415–418, 10.1002/anie.201106154

798 In Vivo Incorporation of Multiple Noncanonical Amino Acids into Proteins N. Budisa, M. G. Hoesl Angewandte Chemie International Edition 2011, 50, 2896–2902, 10.1002/anie.201005680

797 In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids N. Budisa, L. Merkel, S. Lepthien Angewandte Chemie International Edition 2010, 49, 5446–5450, 10.1002/anie.201000439